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Univ of Tennessee
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Stewart

Selected Refereed Manuscripts

Stewart, C.N., Jr. (Ed.) 2008. Plant Biotechnology and Genetics: Principles, Techniques and Applications, Wiley and Sons, New York, 400 pp. June 2008.

Yuan, J.S., D. W. Galbraith, S. Y. Dai, P. Griffin and C.N. Stewart, Jr. 2008. Plant systems biology comes of age. Trends in Plant Science. 13:165-171

Mazarei, M.H., I. Teplova, M. R. Hajimorad and C.N. Stewart, Jr. 2008. Pathogen phytosensing: plants to report plant pathogens. Sensors. 8:2628-2641.

Mazarei, M.H., H. Al-Ahmad, M. R. Rudis and C.N. Stewart, Jr. 2008. Protoplast isolation and transient gene expression in switchgrass, Panicum virgatum L. Biotechnology Journal. 3:354-359.

Burris, K.A., A. Mentewab, S. Ripp, and C.N. Stewart, Jr. 2008. An Arabidopsis thaliana ABC transporter that confers kanamycin resistance in transgenic plants does not endow resistance to Escherichia coli. Microbial Biotechnology. 1:191-195.

Stewart, C.N., Jr. and L. E. Edwards. 2008. How to teach research ethics. The Scientist. 22:27.

Yuan, J., D. Wang. and C. N. Stewart, Jr. 2008. Statistical methods for efficiency adjusted real-time PCR analysis. Biotechnology Journal. 3:112-123.

Yancey, A. and C. N. Stewart, Jr. 2007. Are university researchers at risk for patent infringement? Nature Biotechnology. 25:1225-1228.

Yuan, J.S., J. Burris, N. R. Stewart, A. Mentewab and C. N. Stewart, Jr. 2007. Statistical tools for transgene copy number estimation based on real-time PCR. BMC Bioinformatics. 8(Suppl 2): S6 doi:10.1186/1471-2105-8-S7-S6.

Panthee, D.R., J. Yuan, D.L. Wright, J.J. Marois, D. Mailhot and C. N. Stewart, Jr. 2007. Gene expression analysis in soybean in response tothe casual agent of Asian soybean rust Phakospora pachyrhizi Sydow) in an early growth stage. Functional and Integrative Genomics. 7:291-301. Appels Figure

Moon, H.S., M.D. Halfhill, L. L. Good, P. L. Raymer and C. N. Stewart, Jr. 2007. Characterization of directly transformed weedy Brassica rapa and introgressed B. rapa with Bt crylAc and gfp genes. Plant Cell Reports. 26:1001-1010.

Luo, K., H. Duan, D. Zhao, X. Zheng, W. Deng, Y. Chen, C. N. Stewart, Jr., R. McAvoy, X. Jiang, Y. Wu, A. He, Y. Pei and Y. Li. 2007. 'GM-gene-deletor': fused loxP-FRT recognition sequences dramatically improve the efficiency of FLP or CRE recombinase on transgene excision from pollen and seed of tobacco plants. Plant Biotechnology Journal. 5:263-274.

Stewart, C.N., Jr. 2007. Biofuels and biocontainment. Nature Biotechnology. 25:283-284.

Halfhill, M.D., L.L. Good, C. Basu, J. Burris, C.L.Main, T.C. Mueller, and C.N. Stewart, Jr. 2007. Transformation and segregation of GFP fluorescence and glyphosate resistance in horseweed (Conyza canadensis) hybrids. Plant Cell Reports. 26:303-311.

  • Yuan, J.S., P.J. Tranel, and C.N. Stewart, Jr. 2007. Non-target site herbicide resistance: a family business. Trends in Plant Science. 12:6-13.

  • Darbani, B., A. Eimanifar, C.N. Stewart, Jr., W. Camargo. 2007. Methods to produce marker-free transgenic plants. Biotechnology Journal 2:83-90.

  • Moon, H.S, M.D. Halfhill, L.C. Hudson, R.J. Millwood, and C.N. Stewart, Jr. 2006. Expression of green fluorescent protein in pollen of oilseed rape (Brassica napus L.) and its utility for assessing pollen movement in the field. Biotechnology Journal 1:1147-1152.

  • Ferry, N., E.A. Mulligan, C.N. Stewart, B.E. Tabashnik, G.R. Port, and A.M.R. Gatehouse. 2006. Prey-mediated effects of transgenic canola on a beneficial, non-target, carabid beetle. Transgenic Research 15:501-514.

  • Yuan, J.S., A. Reed, F. Chen, C.N. Stewart, Jr. 2006. Statistical analysis of real-time PCR data. BMC Bioinformatics 7:85.

  • Stewart, C.N., Jr. 2006. Go with the glow: fluorescent proteins to light transgenic organisms. Trends in Biotechnology 24:155-162.

  • Markham, K., T. Chalk, and C.N. Stewart, Jr. 2006. Evaluation of protein-based defenses against phytophagous insects in ferns and mosses. International Journal of Plant Science 167:111-117.

  • Stewart, C.N., Jr., R.J. Millwood, M.D. Halfhill, M. Ayalew, V. Cardoza, M. Kooshki, G.A.Capelle, K.R. Kyle, D. Piaseki, G. McCrum, J. Di Benedetto. 2005. Laser-induced fluorescence imaging and spectroscopy of GFP transgenic plants. Journal of Fluorescence. 15:697-705.

  • Wei, W., T.H. Schuler, S.J. Clark, C.N. Stewart, Jr., and G. M. Poppy. 2005. Age-related increase in levels of insecticidal protein in the progenies of transgenic oilseed rape and its efficacy against a susceptible strain of diamondback moth. Annals of Applied Biology 147:227-235.

  • Mentewab, A. and C. N. Stewart, Jr. 2005. Overexpression of an Arabidopsis thaliana ABC transporter confers kanamycin resistance to transgenic plants. Nature Biotechnology. 23:1177-1180. Supplement


  • Halfhill, M.D.; Sutherland, J.P.; Moon, H.S.; Poppy, G.M.; Warwick, S.I.; Weissinger, A.K.; Rufty, T.W.; Raymer, P.L. and C. Neal Stewart, Jr. 2005. Growth, productivity, and competitiveness of introgressed weedy Brassica rapa hybrids selected for the presence of Bt cry1Ac and gfp transgenes. Molecular Ecology. 14:3177-3189.

  • C. Neal Stewart, Jr. 2005. Monitoring the presence and expression of transgenes in living plants. TRENDS in Plant Science. . 10(8):390-396.

  • Schuler, T. H.; Clark, A.J.; Clark, S. J.; Poppy, G.M.; C. N. Stewart, Jr. and I. Denholm 2005. Laboratory studies of the effects of reduced prey choice caused by Bt plants on a predatory insect. Bulletin of Entomological Research. 95:243-247.

  • Warwick, S.I. and C. N. Stewart, Jr. 2005. Crops Come from Wild Plants - Domestication, Transgenes, and Linkage Together Shape Ferality. pp 9-30 In: J. Gressel (Ed.) Crop Ferality and Volunteerism. CRC Press, Boca Raton, Florida.

  • Mentewab A..; V. Cardoza and C. N. Stewart, Jr. 2005. Genomic analysis of the response of Arabidopsis thaliana to trinitrotoluene as revealed by cDNA microarrays. Plant Science. 168:1409-1424.

  • Cardoza, V. and C. N. Stewart, Jr. 2004. Brassica Biotechnology: progress in cellular and molecular biology. In Vitro Cell. Dev. Biol. - Plant 40:542-551.

  • Halfhill, M.D., R. J. Millwood, and C. N. Stewart, Jr. 2004. GFP Quantification in whole plants. pp. 215-226. In: L. Pena (Ed). Transgenic Plants: Methods and Protocols. Methods in Molecular Biology. Humana Press, Totawa, NJ.

  • Hudson, L.C., M.D. Halfhill, and C. N. Stewart, Jr. 2004. Transgene dispersal through pollen. pp. 365-374 In: L. Pena (Ed). Transgenic Plants: Methods and Protocols. Methods in Molecular Biology. Humana Press, Totawa, NJ.

  • Basu C, Halfhill MD, Mueller TC, Stewart CN. 2004. Weed genomics: new tools to understand weed biology. Trends in Plant Science 9: 391-398.

  • Halfhill MD, Zhu B, Warwick SI, Raymer PL, Millwood RJ, Weissinger AK, Stewart CN. 2004. Hybridization and backcrossing between transgenic oilseed rape and two related weed species under field conditions. Environ. Biosafety Res. 3: 73-81.

  • Cardoza V and Stewart, C.N., Jr.. 2004. Agrobacterium-mediated transformation of canola. pp 379-387 In: I. S. Curtis (Ed.) Transgenic Crops of the World - Essential Protocols Kluwer Academic Publishers, Dordrecht.

  • Patel N, Cardoza V, Christensen E, Rekapalli B, Ayalew M, Stewart, CN. 2004. Differential gene expression of Chlamydomonas reinhardtii in response to 2,4,6-trinitrotoluene (TNT) using microarray analysis. Plant Sciences 167: 1109-1122.

  • Stewart, C.N., Jr. 2004. Genetically Modified Planet: Environmental Impacts of Genetically Engineered Plants. Oxford University Press, New York. 240 pp.

  • Zhu, B., Lawrence, J.R., Warwick, S.I., Mason, P., Braun, L., Halfhill, M.D., and Stewart, C.N. Jr. 2004. Inheriteance of GFP-Bt transgenes from Brassica napus in backcrosses with three wild B. rapa accessions. Environ. Biosafety Res. 3:45-54.

  • Hudson, L.C., and Stewart, C.N. Jr. 2004. Effects of pollen-synthesized green fluorescent protein on pollen grain fitness. Sex Plant Reprod. 17:49-53.

  • Zhu, J., Lawrence, S.I., Warwick, P. Mason, Braun, L., Halfhill, M.D., and Stewart, C. N. Jr. 2004. Stable Bacillus thuringiensis (Bt) toxin content in interspecific F1 and backcross populations of wild Brassica rapa after Bt gene transfer. Molecular Ecology. 13:237-241.

  • Mason, P., Braun, L., Warwick, S. I., Zhu, B., and Stewart, Jr. C.N. 2003. Transgenic Bt-producing Brassica napus: Plutella xylostella selection pressure and fitness of weedy relatives. Environ. Biosafety Res. 2:263-276.

  • Halfhill, M.D., Millwood, R.J., Rufty, T.W., Weissinger, A.K. and Stewart, C.N. Jr. 2003. Spatial and temporal patterns of green fluorescent protein (GFP) fluorescence during leaf canopy development in transgenic oilseed rape, Brassica napus L. Plant Cell Rep. 22:338-343.

  • Halfhill, M.D., Millwood, R.J., Weissinger, Warwick, S.I., Stewart, C. N. 2003. Additive transgene expression and genetic introgression in multiple green-fluorescent protein transgenic crop x weed hybrid generations. Theor Appl. Genet. 107:1533-1540.

  • Stewart, C. Neal, Jr., Halfhill, M.D., Warwick, S.I. 2003. Transgene introgression from genetically modified crops to their wild relatives. Nature Reviews Genetics. 4:806-817.

  • Warwick, S.I., Simard, M.J., Legre, A., Beckie, H.J., Braun, L., Zhu, B., Mason, P., Seguin-Swartz, G., Stewart, C. N. Jr. 2003. Hybridization between transgenic Brassica napus L. and its wild relatives: Brassica rapa L., Raphanus raphanistrum L., Sinapis arvensis L., and Erucastrum gallicum (Willd.) O. E. Schulz. Theor Appl Genet. 107:528-539.

  • Richards, H.A., Halfhill, M.D., Millwood, R.J., Stewart, C. N. Jr. 2003. Quantitative GFP fluorescence as an indicator of recombinant protein synthesis in transgenic plants. Plant Cell Rep. 22:117-121.

  • Kooshki, M., Mentewab, A., Stewart, C. Neal, Jr. 2003. Pathogen inducible reporting in transgenic tobacco using a GFP construct. Plant Science. 165:213-219.

  • Mueller, T.C., Massey, J.H., Hayes, R.M., Main, C.L., and Stewart, C.N. Jr. 2003. Shikimate accumulates in both glyphosate-sensitive and glyphosate-resistant horseweed (Conyza canadensis L. Cronq).J. Agric. Food. Chem. 51:680-684.

  • Schuler, T.H., Potting, R.P.J., Denholm, I., Clark, S.J., Clark, A.J., Stewart, C.N. Jr., and Poppy, G.M. 2003. Tritrophic choice experiments with Bt plants, the diamondback moth (Plutella xylostella) and the parasitoid Cotesia plutellae . Transgenic Research. 12:351-361.

  • Stewart, C. N. Jr. 2003. Press before paper-when media and science collide. Nature Biotechnology. 21:353-354.

  • Stewart, C.N., Jr. 2003. Transgenic Plants: Current Innovations and Future Trends (Editor). Horizon Scientific Press. Wymondham, UK. 297 pp.

  • Millwood, R.J., M.D. Halfhill, D. Harkins, R. Rusotti, and C.N. Stewart, Jr. 2003. Instrumentation and methodology for quantifying GFP fluorescence in intact plant organs . BioTechniques. 34:638-643.

  • Cardoza, V. and C.N. Stewart, Jr. 2003. Increased Agrobacterium-mediated transformation and rooting efficiencies in canola Brassica napus L. from hypocotyl segment explants. Plant Cell Reports 21:599-604.

  • Stewart, C.N., Jr. 2003. (Ed.) Transgenic Plants: Current Innovations and Future Trends. Horizon Scientific Press. Wymondham, UK. 297 pp.

  • Richards, H. A., Han, C. T., Hopkins, R. G., Failla, M. L., Ward, W. W. and Stewart, C.N. Jr. 2003. Safety assessment of recombinant green fluorescent protein orally administered to weaned rats. J. Nutr. 133:1909-1912.

  • Tomlin, E.S., S.R. Branch, D. Chamberlain, H. Gabe, M.S. Wright, and C.N. Stewart, Jr. 2002. Screening of soybean, Glycine max (L.) Merrill, lines for somatic embryo induction and maturation capability from immature cotyledons. In Vitro Cell. Dev. Biol. - Plant. 38:543-548.

  • Halfhill, M.D., R.J. Millwood, P.L.Raymer and C.N. Stewart, Jr. 2002. Bt-transgenic oilseed rape hybridization with its weedy relative, Brassica rapa. Environ. Biosafety Res. 1:19-28.

  • Stewart, C.N., Jr., M. D. Halfhill, and R. J. Millwood. 2002. Geen fluorescent protein in transgenic plants: Brassica transformation. Methods in Molecular Biology. 183(19):245-252.

  • Stewart, C.N., Jr., and S. K. Wheaton. 2002. Urban myths and real risks of genetically enhanced (GE) crops. pp. 528-551. In: Plants, Genes, and Crop Biotechnology. Maarten J. Chrispeels and David E. Sadova, (Eds) Bartlett and Jones Publishers, Sudbury, Mass.

  • Pulliam, D.A., D.L. Williams, R.M. Broadway, and C.N. Stewart, Jr. 2001. Isolation and characterization of a serine proteinase inhibitor cDNA from cabbage and its antibiosis in transgenic tobacco plants. Plant Cell Biotechnology and Molecular Biology. 2 (1&2):19-32.

  • Halfhill, M.D., H.A. Richards, S.A. Mabon, C.N. Stewart, Jr. 2001. Expression of GFP and Bt transgenes in Brassica napus and hybridization and introgression with Brassica rapa. Theoretical and Applied Genetics 103:659-667.

  • Hudson, L.C., D. Chamberlain, and C.N. Stewart, Jr. 2001. GFP-tagged pollen to monitor gene flow of transgenic plants. Molecular Ecology Notes 1:321-324.

  • Stewart, C. Neal Jr., Matthew D. Halfhill, Harold A. Richards. 2001. GFP in plant biotechnology and agriculture, pp. 453-456 in Bioluminescence and Chemiluminescence 2000, J F Case, PJ Herring, BH Robison, SHD Haddock, LJ Kricka, PE Stanley (Eds). World Scientific Publishing Company, Singapore.

  • Stewart, C.N., Jr. 2001. The utility of green fluorescent protein in transgenic plants. Plant Cell Reports 20:376-382.

  • Stewart, C.N., Jr. 2001. Update to applications of green fluorescent protein in plants, pp. 144-145 in Q. Lu and M.P. Weiner (Eds.), Cloning and Expression Vectors for Protein Studies. Eaton Publishing, Westborough, Mass.

  • Stewart, C.N., Jr., and S.K. Wheaton. 2001. GM crop data-agronomy and ecology in tandem. Nature Biotechnology 19:3.

  • Stewart, C.N., Jr., H.A. Richards, M.D. Halfhill. 2000 Transgenic plants and biosafety: science, misconceptions and public perceptions. BioTechniques 29:832-843.

  • Harper, B.K., and C.N. Stewart, Jr. 2000. Patterns of green fluorescent protein in transgenic plants. Plant Molecular Biology Reporter 18: 141a-141i.

  • Ramachandran, S., G.D. Buntin, J.N. All, P.L. Raymer and C.N. Stewart, Jr. 2000. Intraspecific competition of an insect-resistant transgenic canola in seed mixtures. Agronomy Journal 92:368-374.

  • Harper, B.K., S.A. Mabon, S.M. Leffel, M.D. Halfhill, H.A. Richards, K.A. Moyer, and C.N. Stewart, Jr. 1999. Green fluorescent protein in transgenic plants indicates the presence and expression of a second gene. Nature Biotechnology 17:1125-1129.

  • Chamberlain, D and C.N. Stewart, Jr. 1999. Transgene escape and transplastomics. Nature Biotechnology 17:330-331 Invited Commentary.

  • Stewart, C.N., Jr. 1999. Insecticidal transgenes into nature: gene flow, ecological effects, relevancy and monitoring. Pp: 179-190 in, Symposium Proceedings No. 72 Gene Flow and Agriculture-Relevance for Transgenic Crops (ed P.J.W. Lutman) Proceedings of a Symposium held at the University of Keele, 12-14 April 1999. British Crop Protection Council, Surrey, UK.

  • Ramachandran, S., G.D. Buntin, J.N. All, B.E. Tabashnik, P.L. Raymer M.J. Adang, D.A. Pulliam, and C.N. Stewart, Jr. 1998. Survival, fitness, and oviposition of resistant diamondback moth (Lepidoptera: Plutellidae) on transgenic canola producing a Bacillus thuringiensis toxin. Journal of Economic Entomology 91:1239-1244.

  • Ramachandran, S., G.D. Buntin, J.N. All, P.L. Raymer, and C.N. Stewart, Jr. 1998. Movement and survival of diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae) larvae in mixtures of non-transgenic and transgenic canola containing a Bacillus thuringiensis cryIA(c) gene. Environmental Entomology 27:649-656.

  • Ramachandran, S., G.D. Buntin, J.N. All, P.L. Raymer, and C.N. Stewart, Jr. 1998. Greenhouse and field evaluations of transgenic canola against diamondback moth, Plutella xylostella and corn earworm, Helicoverpa zea. Entomologia Experimentalis et Applicata 88:17-24.

  • Stewart, C.N., Jr. 1997. Rapid DNA extraction from plants. pp. 25-28 in: M.R. Micheli and R. Bova (eds.) Fingerprinting Methods Based on Arbitrarily Primed PCR, Springer Verlag, Heidelberg.

  • Leffel, S.M., S.A. Mabon, and C.N. Stewart, Jr.1997. Applications of green fluorescent protein in plants. BioTechniques 23: 912-918.

  • Stewart, C.N., Jr., J.N. All, P.L Raymer, S. Ramachandran. 1997. Increased fitness of transgenic insecticidal rapeseed under insect selection pressure. Molecular Ecology 6:773-779.

  • Stewart, C.N., Jr. 1996. Transgene flow and persistence may be monitored using in vivo markers such as GFP. Biosafety Volume 2, (BY96003), September 2nd 1996. Refereed Online Journal, URL - http://www.bdt.org.br/bioline/by.

  • Stewart, C.N., Jr. 1996. Monitoring transgenic plants with in vivo markers. Nature Biotechnology 14:682.


  • Stewart, C.N., Jr., M.J. Adang, J.N. All, H.R. Boerma, G. Cardineau, and W.A. Parrott. 1996. Genetic transformation, recovery, and characterization of transgenic soybean for synthetic Bacillus thuringiensis cryIA(c). Plant Physiology 112:121-129.

  • Stewart, C.N., Jr., M.J. Adang, J.N. All, P.L Raymer, S. Ramachandran, and W.A. Parrott. 1996. Insect control and dosage effects in transgenic canola, Brassica napus L. (Brassicaceae), containing a synthetic Bacillus thuringiensis cryIA(c) gene. Plant Physiology 112:115-120.

  • Stewart, C.N.,Jr., and L. Excoffier. 1996. Assessing population genetic structure and variability with RAPD data: application to the American cranberry. Journal of Evolutionary Biology 9:153-171.

  • Stewart, C.N., Jr., G. Rosson, B.W. Shirley, and D.M. Porter. 1996. Population genetic variation of rare and endangered Iliamna (Malvaceae) in Virginia. Biological Journal of the Linnean Society 58:357-369.

  • Stewart, C.N., Jr., and E.T. Nilsen. 1995. Phenotypic plasticity and genetic variation of Vaccinium macrocarpon (American cranberry) I. Reaction norms of clones from central and marginal populations in a common garden. International Journal of Plant Sciences 156:687-697.

  • Stewart, C.N., Jr., and E.T. Nilsen. 1995. Phenotypic plasticity and genetic variation of Vaccinium macrocarpon (American cranberry) II. Reaction norms and spatial clonal patterns in two marginal populations. International Journal of Plant Sciences 156:698-708.

  • Stewart, C.N., Jr., and D.M. Porter. 1995. The usefulness of RAPD profiling in biological conservation: an application to estimating clonal variation in rare and endangered Iliamna in Virginia. Biological Conservation 74:135-142.

  • Parrott, W.A., J.N. All, M.J. Adang, M.A. Bailey, H.R. Boerma, and C.N. Stewart, Jr. 1994. Recovery and evaluation of soybean (Glycine max [L.] Merr.) plants transgenic for a Bacillus thuringiensis var. kurstaki insecticidal gene. In Vitro Cellular and Developmental Biology-Plant. 30:144-149.

  • Stewart, C.N., Jr., and E.T. Nilsen. 1993. Association of edaphic factors and vegetation in several isolated Appalachian peat bogs. Bulletin of the Torrey Botanical Club 120:128-135.

  • Stewart, C.N., Jr., and E.T. Nilsen. 1993. Responses of Drosera capensis and D. binata var. multifida (Droseraceae) to manipulations of insect availability and soil nutrient levels. New Zealand Journal of Botany 31:385-390.

  • Stewart, C.N., Jr., and L.E. Via. 1993. A rapid CTAB DNA isolation technique useful for RAPD fingerprinting and other PCR applications. BioTechniques 14:748-751.

  • Stewart, C.N., Jr., and E.T. Nilsen. 1992. Drosera rotundifolia growth and nutrition in a natural population with special reference to the significance of insectivory. Canadian Journal of Botany 70:1409-1416.

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