Neal Stewart's Current Team Members:

Reggie Millwood Research Scientist rmillwood@utk.edu Education: University of North Carolina, Greensboro 1998 BA Biology, Concentration in Environmental Science and Biotechnology. Research Interests: Currently, I am utilizing functional genomics tools, such as cDNA microarray analysis, to ascertain the genomic responses of Chlamydomonas reinhardtii in the presence of xenobiotics. The goal is to determine whether this particular green algae can be used as a biosensor for chemical warfare agents and explosives. I plan to employ the green fluorescent protein (GFP) as a visual marker for this project. I previously have used GFP to monitor gene flow from crop plants to weeds and to explore the effects of additive transgene expression. In addition, I am developing a model system in Brassica napus to further study additive transgene expression. Outside of work, I enjoy spending most of my time with my wife and our two sons. I enjoy coaching my oldest son's sports teams, playing golf, reading, playing golf, watching UNC basketball (as well as UT basketball), and finally, playing golf. Articles: Halfhill, M.D., B. Zhu, S.I. Warwick, P.L. Raymer, R.J. Millwood, A.K. Weissinger, C. N. Stewart, Jr. 2004. Gene flow from transgenic oilseed rape and crop x weed hybrids under field conditions. Environmental Biosafety Research, 3:1-9. Halfhill, M.D., R.J. Millwood, A.K. Weissinger, S.I. Warwick, and C.N. Stewart, Jr. 2003. Additive transgene expression and genetic introgression in multiple GFP transgenic crop x weed hybrid generations. Theoretical and Applied Genetics, 107:1533-1540. Halfhill, M.D., R.J. Millwood, T.W. Rufty, A.K. Weissinger, C.N. Stewart, Jr. 2003. Spatial and temporal patterns of green fluorescent protein (GFP) fluorescence during leaf canopy development in transgenic oilseed rape, Brassica napus L. Plant Cell Reports, 22:338-343. Richards, H.A., M.D. Halfhill, R.J. Millwood, and C.N. Stewart, Jr. 2003. Quantitative GFP fluorescence as an indicator of recombinant protein synthesis in transgenic plants. Plant Cell Reports, 22:117-121. Millwood, R. J., M. D. Halfhill, D. Harkins, R. Russotti, and C. N. Stewart, Jr. 2003. Instrumentation and methodology for quantifying GFP fluorescence in intact plant organs. BioTechniques, 34: 638-643. Stewart, C.N. Jr., M.D. Halfhill, R.J. Millwood, P.L. Raymer. 2003. Cross-breeding modified crops. Letter to the editor. New Scientist. Jan, 11 (web version http://www.newscientist.com/opinion/opletters.jsp?id=ns237719). Halfhill, M.D., R.J. Millwood, P.L. Raymer, C.N. Stewart, Jr. 2002. Bt-transgenic oilseed rape hybridization with its weedy relative, Brassica rapa. Environmental Biosafety Research, 1:19-28. Book Chapters: Halfhill, M.D., Millwood, R.J., Stewart, C.N., Jr. (2004). GFP quantification in whole plants. In: Methods in Molecular Biology: Green Fluorescent Protein: Applications and Protocols. Hicks, BW (Ed.), Humana Publishing. Totowa, NJ. 215-225. Stewart, C.N., Jr., Halfhill, M.D., Millwood, R.J. (2002). Green fluorescent protein in transgenic plants: Brassica transformation. In: Methods in Molecular Biology, vol. 183: Green Fluorescent Protein: Applications and Protocols. Hicks, BW (Ed.), Humana Publishing. Totowa, NJ. 245-252. Millwood, R.J., Halfhill, M.D., and Stewart, C.N., Jr. (2001). Visualization of GFP transgenic plant cells from undifferentiated tissue to shoots, GFP in Motion II CD. Supplement to Trends in Cell Biology.