Green CD, Stodola AJW, Augé RM. 1998. Transpiration of detached leaves from mycorrhizal and nonmycorrhizal cowpea and rose plants given varying abscisic acid, pH, calcium and phosphorus. Mycorrhiza 8: 93-99. Vesicular-arbuscular mycorrhizal (VAM) colonization can alter transpiration of host leaves, but scientists remain unclear about the mechanisms involved. We tested whether intact root systems were required to observe effects of root colonization by Glomus intraradices on leaf transpiration, or whether some VAM influence resided in leaves even after they were detached from root systems. We measured transpiration of detached leaves of VAM and nonmycorrhizal (NM) plants at different levels of several substances known to influence stomata locally or act in whole plant regulation of transpiration: abscisic acid, calcium, phosphorus and hydrogen ions. In rose, some VAM influence on transpiration resided in leaves, even after they were separated from their root systems. However, removing leaves from their root systems eliminated the VAM influence on stomatal behavior of cowpeas.